棉花生物学国家重点实验室

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研究队伍

刘方

1、个人简历
    刘方,男,博士、研究员、博士生导师,主要从事棉花野生资源收集保存与研究利用。在棉花种质资源收集鉴定评价、棉花细胞与分子生物学、棉花基因克隆与遗传转化等方面做出突出成绩。先后主持并参与国家转基因重大专项、973计划、863计划、国家自然基金、948项目、棉花产业体系、农业部保种、国家科技支撑计划等国家、重点研发计划等项目30余项,在国内外核心期刊发表科研论文80余篇,申报发明专利20余件,获国家科技进步二等奖1项,获省部级科技进步奖6项,年科研经费100余万元。

2、教育经历   
2013-2016,西南大学,作物遗传育种专业 博士
2006-2009,中国农业科学院,作物遗传育种专业 硕士
1992-1996,河南大学,生物学专业 学士

3、工作经历
1996-至今,中国农业科学院棉花研究所生物技术研究室
2010-2011,美国罗格斯大学及阿肯色大学,访问学者

4、承担项目
(1)国家转基因重大专项, 2008ZX2016-003-005、优质纤维转基因棉花新品种培育、2016/01-2020/12、5600万、子课题主持
(2)国家自然基金面上项目、31671745、陆地棉野生种系产量性状有利等位基因挖掘、2017/01-2020/12,62万元、主持
(3)国家科技基础条件平台项目、NICGR2016-034, 野生棉种质资源子平台、20万,2017,主持
(4)国家863项目、2013AA102601-01-11、棉花耐旱碱功能基因研究、2013/01-2017/12、50万元、子课题主持
(5)国家863项目、2012AA101108-02、四倍体野生棉遗传连锁图谱构建、2012/01-2015/12、5万元、子课题主持
(6)国家973项目、2010CB126000、四倍体野生棉优异基因源挖掘与QTL定位研究、2010/01-2014/12、50万元、子课题主持
(7)农业部保种项目、2014NWB034、野生棉种质资源繁殖更新与利用、2014/01-2014/12、23万元、主持
(8)国家948项目、2011-G1-04、国外优异和特色棉花种质资源引进与利用、2011/01-2015/12、100万元、子课题主持
(9)国家948项目、课题主持
(10)转基因重大专项、2009ZX08005016B、转基因抗除草剂棉花新材料创制研究、2011/01-2012/12、265万元、课题主持

5、代表论文与著作
(1)Richard Odongo, Magwang, Pu Lu, Joy Nyangasi Kirungu, Hejun Lu, Xingxing Wang, Xiaoyan Cai1, Zhongli Zhou, Zhenmei Zhang, Haron Salih, Kunbo Wang and Fang Liu, Characterization of the late embryogenesis abundant (LEA) proteins family and their role in drought stress tolerance in upland cotton, BMC Genetics, 2018, 19:6, DOI 10.1186/s12863-017-0596-1.
(2)Joy Nyangasi Kirungu, Yanfeng Deng, Xiaoyan Cai, Richard Odongo Magwanga, Zhongli Zhou, Xingxing Wang , Yuhong Wang, Zhenmei Zhang, Kunbo Wang and Fang Liu, Simple Sequence Repeat (SSR) Genetic Linkage Map of D Genome Diploid Cotton Derived from an Interspecific Cross between Gossypium davidsonii and Gossypium klotzschianum, International Journal of Molecular Sciences, 2018, 19, 204; doi:10.3390/ijms19010204.
(3)Pu Lu, Richard Odongo Magwanga, Hejun Lu, Joy Nyangasi Kirungu, Yangyang Wei , Qi Dong, Xingxing Wang , Xiaoyan Cai, Zhongli Zhou, Kunbo Wang and Fang Liu, A Novel G-Protein-Coupled Receptors Gene from Upland Cotton Enhances Salt Stress Tolerance in Transgenic Arabidopsis, genes, 2018, 9, 209; doi:10.3390/genes9040209
(4)Ying Wu, Fang Liu, Dai-Gang Yang, Wei Li, Xiao-Jian Zhou, Xiao-Yu Pei, Yan-Gai Liu, Kun-Lun He, Wen-Sheng Zhang, Zhong-Ying Ren, Ke-Hai Zhou, Xiong-Feng Ma and Zhong-Hu Li, Comparative Chloroplast Genomics of Gossypium Species: Insights Into Repeat Sequence Variations and Phylogeny, Frontiers in plant Sciences, 2018, 9:376. doi: 10.3389/fpls.2018.00376.
(5)Yuling Liu, Baohong Zhang, Xinpeng Wen, Shulin Zhang, Yangyang Wei, Quanwei Lu, Zhen Liu, Kunbo Wang, Fang Liu, Renhai Peng, Construction and characterization of a bacterial artificial chromosome library for Gossypium mustelinum, Plos ONE, 2018, 13(5): e0196847, https://doi.org/10.1371/journal.pone.0196847
(6)Pu Lu, Richard Odongo Magwanga, Xinlei Guo, Joy Nyangasi Kirungu, Hejun Lu, Xiaoyan Cai, Zhongli Zhou, Yangyang Wei, Xingxing Wang1, Zhenmei Zhang, Renhai Peng, Kunbo Wang and Fang Liu, Genome-wide analysis of Multidrug and toxic compound extrusion ( MATE) family in diploid cotton, G. raimondii and G. arboreum and its expression analysis under salt,  cadmium and drought Stress, G3: Genes|Genomes|Genetics, 2018, doi:10.1534/g3.118.200232
(7)Richard Odongo Magwanga, Pu Lu, Joy Nyangasi Kirungu, Latyr Diouf , Qi Dong, Yangguang Hu, Xiaoyan Cai 1, Yanchao Xu , Yuqing Hou, Zhongli Zhou, Xingxing Wang, Kunbo Wang and Fang Liu, GBS Mapping and Analysis of Genes Conserved between Gossypium tomentosum and Gossypium hirsutum Cotton Cultivars that Respond to Drought Stress at the Seedling Stage of the BC2F2 Generation, International Journal of Molecular Sciences, 2018, doi:10.3390/ijms19061614
(8)Richard Odongo Magwanga, Pu Lu, Joy Nyangasi Kirungu, Qi Dong, Yangguang Hu, Zhongli Zhou, Xiaoyan Cai, Xingxing Wang, Yuqing Hou, Kunbo Wang,  and Fang Liu, Cotton Late Embryogenesis Abundant (LEA2) Genes Promote Root Growth and Confer Drought Stress Tolerance in Transgenic Arabidopsis thaliana, G3: Genes|Genomes|Genetics, 2018, doi: https://doi.org/10.1534/g3.118.200423.
(9)Allah Ditta, Zhongli Zhou, Xiaoyan Cai, XingxingWang , KiflomWeldu Okubazghi, Muhammad Shehzad, Yanchao Xu, Yuqing Hou ,Muhammad Sajid Iqbal, Muhammad Kashif Riaz Khan, KunboWang, and Fang Liu, Assessment of Genetic Diversity, Population Structure, and Evolutionary Relationship of Uncharacterized Genes in a Novel Germplasm Collection of Diploid and Allotetraploid Gossypium Accessions Using EST and Genomic SSR Markers, International Journal of Molecular Sciences, 2018, doi:10.3390/ijms19082401.
(10)Richard Odongo Magwanga, Pu Lu, Joy Nyangasi Kirungu, Wenyun Xiao, Zhongli Zhou, Xiaoyan Cai, Xingxing Wang, Zhenmei Zhang , Kunbo Wang and Fang Liu, Whole Genome Analysis of Cyclin Dependent Kinase (CDK) Gene Family in Cotton and Functional Evaluation of the Role of CDKF4 Gene in Drought and Salt Stress Tolerance in Plants, International Journal of Molecular Sciences, 2018, doi:10.3390/ijms19092625
(11)Allah Ditta, Zhongli Zhou, Xiaoyan Cai, Muhammad Shehzad, Xingxing Wang , Kiflom Weldu Okubazghi, Yanchao Xu, Yuqing Hou, Muhammad Sajid Iqbal, Muhammad Kashif Riaz Khan, Kunbo Wang,  and Fang Liu, Genome-Wide Mining and Characterization of SSR Markers for Gene Mapping and Gene Diversity in Gossypium barbadense L. and Gossypium darwinii G. Watt Accessions, Agronomy, 2018, doi:10.3390/agronomy8090181
(12)邓艳凤,路普,周忠丽,蔡小彦,王星星,王玉红,张振梅,刘方,王坤波, 不同国家和地区海岛棉SSR 遗传多样性分析,分子植物育种,2018,16(1),1-16。
(13)Liu Yuling, Liu Zhen, Peng Reihai, Wang Yuhong, Zhou Zhongli, Cai Xiaoyan, Wang Xingxing, Zhang Zhenmei, Wang Kunbo*, Liu Fang*. Cytogenetic maps of homoeologous chromosomes Ah01 and Dh01 and their integration with the genome assembly in Gossypium hirsutum. Comparative Cytogenetics, 2017, 11(2): 405-420. doi:10.3897/CompCytogen.v11i2.12824(SCI,ISSN1993-0771)
(14)Xueying Liu, Zhonghua Teng, Jinxia Wang, Tiantian Wu, Zhiqin Zhang, Xianping Deng, Xiaomei Fang, Zhaoyun Tan, Iftikhar Ali, Dexin Liu, Jian Zhang, Dajun Liu, Fang Liu*, Zhengsheng Zhang*,Enriching an intraspecific genetic map and identifying QTL for fiber quality and yield component traits across multiple environments in Upland cotton (Gossypium hirsutum L.),Molecular Genetic Genomic,2017,292:1281–1306.
(15)Wen-wen Wang, Zhao-yun Tan, Ya-qiong Xu, Ai-ai Zhu, Yan Li, Jiang Yao, Rui Tian, Xiao-mei Fang, Xue-ying Liu, You-ming Tian, Zhong-hua Teng, Jian Zhang, Da-jun Liu, De-xin Liu, Hai-hong Shang, Fang Liu* & Zheng-sheng Zhang,Chromosome structural variation of two cultivated tetraploid cottons and their ancestral diploid species based on a new high-density genetic map,scientific report,2017,DOI:10.1038/s41598-017-08006-w.
(16)Kiflom Weldu Okubazghi, LI Xiao-na, CAI Xiao-yan, WANG Xing-xing, CHEN Hao-dong, ZHOU Zhong-li, WANG Chun-ying, WANG Yu-hong, LIU Fang*, WANG Kun-bo,Genome-wide assessment of genetic diversity and fiber quality traits characterization in Gossypium hirsutum races,Journal of Integrative Agriculture,2017, 16(11): 2402-2412.
(17)Xinlei Guo, Yuanyuan Wang, Hejun Lu, Xiaoyan Cai, Xingxing Wang, Zhongli Zhou, Chunying Wang, Yuhong Wang, Zhenmei Zhang, Kunbo Wang, Fang Liu*,Genome-wide characterization and expression analysis of the aldehydedehydrogenase (ALDH) gene superfamily under abiotic stresses in cotton,gene,2017,628 (2017) 230–245.
(18)Yangyang Wei, Yanchao Xu, Pu Lu, Xingxing Wang, Zhenqing Li, Xiaoyan Cai, Zhongli Zhou, Yuhong Wang, Zhenmei Zhang, Zhongxu Lin, Fang Liu*, Kunbo Wang,Salt stress responsiveness of a wild cotton species (Gossypium klotzschianum) based on transcriptomic analysis,PLOS ONE,2017,e0178313.https://doi.org/10.1371/journal. pone.0178313.
(19)Xiao Shuiping, Wang Kunbo, Yang Lei, Ke Xingsheng, Wang Chunying, Liu Xinwen, Sun Liangqing, Yang Shaoqun, Liu Fang*, Chen Yi,Analyses on Progenitor Donors of the Cultivated Allotetraploid Cottons Revealed by GISH,Molecular Plant Breeding,2016,7(14): 1-10.
(20)Juyun Zheng, Oluoch George, Muhammad Kashif Riaz Khan, Xingxing Wang, Xiaoyan Cai, Zhongli Zhou, Chunying Wang, Yuhong Wang, Xueyuan Li, Fang Liu* and Kunbo Wang,Mapping QTLs for drought tolerance in an inter-specific F2:3 population including the wild cotton species gossypium tomentosum,Genetics and Molecular Research,2016: 15(3),DOI: 10.4238/gmr.15038477
(21)George Oluoch, Juyun Zheng, Xingxing Wang, Muhammad Kashif Riaz Khan, Zhongli Zhou, Xiaoyan Cai, Chunying Wang, Yuhong Wang, Xueyuan Li . Heng Wang . Fang Liu . Kunbo Wang,QTL mapping for salt tolerance at seedling stag in the interspecific cross of Gossypium tomentosum with Gossypium hirsutum,Euphytica,2016,209: 223-235
(22)Yuan-Yuan Wang,  Zhong-Li Zhou, Xing-Xing Wang, Xiao-Yan Cai, Xiao-Na Li, Chun-Ying Wang , Yu-Hong Wang , Liu Fang*, Kun-Bo Wang,Genome-wide association mapping of glyphosate-resistance in Gossypium hirsutum races,Euphytica,2016,209:209-221.
(23)M Kashif Riaz Khan, Zhongli Zhou,  Haodong Chen, M. Kashif Ilyas, Xingxing Wang, Xiaoyan Cai1, ChunyingWang, Zhong Li Wang, Fang Liu*, Kunbo Wang,Genome Wide SSR High Density Genetic Map Construction from an Interspecific Cross of Gossypium hirsutum × Gossypium tomentosum, Frontiers in Plant Science, 2016,7(436): 1-17.
(24)F. Liu, Z. L. Zhou, C. Y. Wang, Y. H. Wang, X. Y. Cai, X. X. Wang, K. B. Wang* & Z. S. Zhang*,Collinearity Analysis of Allotetraploid Gossypium Tomentosum and Gossypium Darwinii,Genetics and Molecular Research,2016: 15(3),DOI: 10.4238/gmr.15038391.
(25)Liu, F.,Wang, Y. H.,Gao, H. Y.,Wang, C. Y.,Zhou, Z. L.,Cai, X.Y.,Wang, X. X.,Zhang, Z. S.,Wang, K. B.*,Construction and characterization of a bacterial artificial chromosome library for the allotetraploid Gossypium tomentosum,Genetics and Molecular Research,2016,14(4):16975-16980.
(26)F. Liu, Z. L. Zhou, C. Y. Wang, Y. H Wang, X. Y. Cai, X. X. Wang, Z. S. Zhang, K. B. Wang* , Genetic Diversity and Relationship Analysis of Gossypium arboreum accession. Genetics and Molecular Research,2015,14 (4): 14522-14529.

6、专利
(1)刘玉玲,赵艳艳,刘方,周忠丽,蔡小彦,王星星,王春英,王玉红,彭仁海,王坤波。棉属AD或ADE背景下同时鉴别各亚组全套染色体的BAC-FISH方法,,2017年3, ZL201510099093.2
(2)崔兴雷, 刘玉玲, 刘方, 王星星, 蔡小彦, 彭仁海, 周忠丽, 王春英, 王玉红, 王坤波。FISH制片再次杂交的方法,2016年7月, 201410534116.3
(3)崔兴雷,刘方,刘玉玲,王省芬,王春英,周忠丽,蔡小彦,王星星,王玉红,马峙英,彭仁海,王坤波。鉴别棉花A genome和A sub-genome全套染色体的方法,2016年2月,ZL201410060083.3
(4)崔兴雷,刘方,刘玉玲,孟菲,王春英,周忠丽,蔡小彦,王星星,王玉红,彭仁海,王坤波。鉴别棉花D genome和D sub-genome全套染色体的方法,2016年3月, ZL201410594840.5
(5)高海燕、王坤波、马峙英、刘方、王省芬、王春英、张香娣、王玉红、彭仁海。利用非暗培养叶片构建棉花BAC文库的方法,,2014年6月, ZL201210215857.6
(6)刘方、张保龙、王坤波、陈天子、蔡小彦。沉默两个抗性基因防治烟灰虱的方法,2014年5月,中国,ZL 2012 1 0025653.6
(7)蔡小彦、王坤波、刘方、王春英、黎绍惠、张香娣、王玉红。棉花叶片抗盐鉴定方法,2013年10月, ZL201210053538.X
(8)王园园、张香娣、王坤波、刘方、王春英、王玉红、黎绍惠、蔡小彦。棉花叶片棉球定位鉴定除草剂抗性的方法,2013年11月, ZL201210019682.1
(9)张保龙、王坤波、杨郁文、刘方、陈天子。棉花表面受体蛋白基因Gbvdr3启动子应用,2013年7月, ZL201210111366.7。

7、获得奖励
(1)“棉属染色体与基因组分析及四倍体棉种演化”获得省科技进步一等奖,2016-J-1-R3/15,2016年
(2)野生棉研究团队获得中华农业科技优秀团队奖(一等奖),2015年
(3)2014年度河南省学术技术带头人,第9412014050号,2015年
(4)“棉花南繁和品种纯度南繁鉴定技术”获得农业部中华农业科技二等奖, KJ2011-G2-007-05,2011年
(5)“南繁棉花的生育规律和关键技术研究”获得海南省科技进步一等奖,证书编号2010-1-1-R-20,2010年
(6)“野生与特色棉花遗传资源的创新与利用研究”获得国家科技进步二等奖,2006-J-201-2-12,2006年

8、联系方式
(1)邮箱:liufcri@163.com
(2)通讯地址:河南安阳黄河大道38号